Vine readiness

‘Premier Russet’ has a late vine maturity and ‘Rio Grande Russet’ has a medium late maturity. 0–6.5) than ‘Premier Russet’ (6.0–7.5). For the progeny, mean trait values displayed a wide range of phenotypic variation (Additional file 4: Table S3), with a high correlation between years (> 0.70, P < 0.001) and across environments (> 0.55, P < 0.001). The broad-sense heritability for both years at each location was statistically significant, with H 2 estimates ranging from 0.83 to 0.93 (Table 2). These results were consistent for all vine maturity traits. When mean values for maturity were mapped to the linkage maps, a significant association was detected on chromosome 5 (Fig. 2, Additional file 6: Figure S2). This association was consistent between years and environments. Logarithm of the odds (LOD) scores ranged from of 21.2 and 34.1, well above the permutation thresholds. Collectively, they explained between 38 and 54% of the trait variance (Table 3). The peak of the LOD profile was consistently detected at 15.0 cM (Table 3, Fig. 2). The best simple model for maturity was a double-simplex configuration (AAAB ? AAAB) on homologous chromosomes H2 and H8 of Rio Grande russet (RGR) and Premier Russet (PR), respectively (Additional file 6: Figure S2). This model implies a QTL with allele effects from both parents, and with the B allele associated with earlier maturity (P < 0.001). Thus, progeny with two copies of the B allele (AABB) had on average earlier maturity (3.1–4.0) than progeny with a single copy (AAAB, 4.5–6.2) or lacking the B allele (AAAA, 6.4–7.5).

LOD get distribution from QTLs getting traits from the maturity locus region on chromosome 5. For each and every panel on the figure means a new characteristic. LOD threshold 95% believe durations are placed in Dining table 3. California, citric acidic 2010; EB, very early blight; FBE, bud-stop fry color Idaho; GH, gains behavior; GLU, glucose; M95, vine readiness from the 95DAP; M120, vine maturity at the 120DAP; TSHA, tuber profile; VW, Verticillium wilt; ID, Idaho; NC, Vermont; DAP, weeks immediately after growing; numbers 10 and you will 11 on the attribute names suggest ages 2010 and you can 2011, respectively

Brand new SNP nearest to your QTL to possess readiness which had good double-simplex configuration are solcap_snp_c2_11605, located at 9.step one cM. Regression of the readiness suggest thinking on genotype regarding the SNP told me anywhere between 38 and you may fifty% of your own phenotypic variance. The newest peak of your LOD contour (fifteen cM) is organized within a gap between markers that spanned 8 so you can 10 cM, to the solcap_snp_c2_11605 found on you to definitely side of the gap. This particular area ports brand new Solanum tuberosum bicycling DOF foundation gene step one (StCDF1, PGSC0003DMG400018408), which had been considered a major regulator of potato plant readiness . Visual image of your genetic and you will real (Mb) range away from homologous chromosomes H2 and you may H8 demonstrated this new asked contour profile and you may fit well with chromosome design proving a high degree out-of concordance within genetic condition (cM) and physical venue (Mb) (Even more document seven: Figure S3).

Multiple attributes co-localize during the readiness locus part with the chromosome 5

7 characteristics including citric acid (CANC10), development routine (GHID, GHNC), bud-prevent fry colour (FBEID), very early blight score (EBID11), tuber sugar (GLUID11), tuber shape (TSHAID), and you can Verticillium wilt get (VWID11) co-surrounding that have vine readiness qualities (MAT95ID, MAT95NC, MAT120ID, MAT120NC) to your chromosome 5 (Dining table 3, Fig. 2). A knowledgeable hereditary model for all faculties, but GLUID11 and you will FBEID was a dual-simplex impact on H2 and you may H8 into the SNP, solcap_snp_c2_11605, that have that it setting (Dining table 3). If residuals from the regression of all of the six faculties to the vine readiness was basically examined, Top Dating-App kostenlos no extreme effectation of a beneficial QTL is receive having EBID11, TSHAID, and you may GHNC. Having progress behavior (GHID) and you can VWID11, a significant QTL is seen only if remedied getting readiness within 95DAP however, was not high from the 120DAP. Data off sugar density (GLUID11) demonstrated a great LOD regarding 3.9 from the 21 cM, that have good QTL affect H2 and you may H5. Immediately following adjusting having maturity, a top in the LOD bend to own GLUID11 is actually noticed just below the 95% permutation endurance.